Effect of short-term macrophage depletion in the development of posterior capsule opacification in rodents


Autoria(s): Lois, N.; Dawson, R.; McKinnon, A.D.; Smith, G.C.; Forrester, J.V.; Townend, J.; Van'T Hof, R.; Van Rooijen, N.
Data(s)

01/11/2008

Resumo

Aim: To evaluate the role of macrophages in the development of posterior capsule opacification (PCO). Methods: For this purpose, an extracapsular lens extraction was performed in 18 consecutive Sprague-Dawley rats. Animals were treated with liposomal clodronate (Cl MDP-lip-treated group, n = 10) or phosphate-buffered saline (PBS) (control group, n = 8) 1 day preoperatively and on the first day postoperatively, and sacrificed 3 days postoperatively. Masked clinical, light microscopy and immunohistochemistry studies were conducted. The Fisher exact test and randomisation test were used to assess statistically differences between groups. Results: A statistically significant reduction in the number of macrophages (ED1+, ED7+, ED8+) was found in the Cl MDP-lip-treated group compared with the PBS-lip-treated group (p = 0.048, p = 0.004, p = 0.027, respectively). There were no statistically significant differences with regards to the presence/absence of central opacification (p = 0.29) and capsular wrinkling (p = 0.21) as detected clinically between groups. Similarly, a qualitative evaluation of the degree of PCO with regards to lens epithelial cell (LEC) proliferation, capsular wrinkling and Soemmerring ring formation showed no statistically significance between groups (p = 0.27, p = 0.061, p = 1.0, respectively). However, a statistically significant reduction in the number of lens epithelial cells (LEC) counted in the centre of the posterior capsule was found in the Cl MDP-lip- treated group (p = 0.009). Conclusion: Depletion of macrophages was accompanied by a reduction in LEC in the centre of the posterior capsule in rodents.

Identificador

http://pure.qub.ac.uk/portal/en/publications/effect-of-shortterm-macrophage-depletion-in-the-development-of-posterior-capsule-opacification-in-rodents(9d842a8a-8e7a-4cff-bc91-355192252232).html

http://dx.doi.org/10.1136/bjo.2007.130518

http://www.scopus.com/inward/record.url?partnerID=yv4JPVwI&eid=2-s2.0-55449120445&md5=9839b93c4a7f32e60eef1f656a73590d

Idioma(s)

eng

Direitos

info:eu-repo/semantics/restrictedAccess

Fonte

Lois , N , Dawson , R , McKinnon , A D , Smith , G C , Forrester , J V , Townend , J , Van'T Hof , R & Van Rooijen , N 2008 , ' Effect of short-term macrophage depletion in the development of posterior capsule opacification in rodents ' British journal of ophthalmology , vol 92 , no. 11 , pp. 1528-1533 . DOI: 10.1136/bjo.2007.130518

Tipo

article