Production and evaluation of the utility of novel phage display-derived peptide ligands to Salmonella spp. for magnetic separation
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12/06/2013
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Resumo |
Aims: The objectives of this study were to produce Salmonella-specific peptide ligands by phage display biopanning and evaluate their use for magnetic separation (MS).<br/>Methods and Results: Four phage display biopanning rounds were performed and the peptides expressed by the two most Salmonella-specific (on the basis of phage binding ELISA results) phage clones, MSal020401 and MSal020417, were chemically synthesized and coupled to MyOne™ tosylactivated Dynabeads®. Peptide capture capability for whole Salmonella cells from non-enriched broth cultures was quantified by MS + plate counts and MS + Greenlight™ detection, and compared to capture capability of anti-Salmonella (antibody-coated) Dynabeads®. MS + Greenlight™ gave a more comprehensive picture of capture capability than MS + plate counts and showed that Peptide MSal020417-coated beads exhibited at least similar, if not better, capture capability to anti-Salmonella Dynabeads® (mean capture values of 36.0 ± 18.2 % and 31.2 ± 20.1 %, respectively, over Salmonella spp. concentration range 3 x 101 - 3 x 106 cfu ml-1) with minimal cross-reactivity (= 1.9 %) to three other foodborne bacteria.<br/>Conclusions: One of the phage display-derived peptide ligands was demonstrated by MS + Greenlight™ to be a viable antibody-alternative for MS of Salmonella spp.<br/>Significance and Impact of Study: This study demonstrates an antibody-free approach to Salmonella detection and opens substantial possibilities for more rapid tests for this bacterium. |
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Idioma(s) |
eng |
Direitos |
info:eu-repo/semantics/restrictedAccess |
Fonte |
Morton , M J , Karoonuthaisiri , N , Stewart , L , Oplatowska , M , Elliott , C & Grant , I 2013 , ' Production and evaluation of the utility of novel phage display-derived peptide ligands to Salmonella spp. for magnetic separation ' Journal of Applied Microbiology , vol 115 , no. 1 , pp. 271--281 . DOI: 10.1111/jam.12207 |
Palavras-Chave | #Salmonella #bacteriophages #peptides #detection #magnetic separation #/dk/atira/pure/subjectarea/asjc/2400/2402 #Applied Microbiology and Biotechnology #/dk/atira/pure/subjectarea/asjc/1300/1305 #Biotechnology |
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article |