The determination of asialoglycoforms of serum glycoproteins by lectin blotting with Ricinus communis agglutinin
Data(s) |
01/02/1992
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Resumo |
Serum proteins were fractionated by polyacrylamide gel electrophoresis under denaturing conditions and transferred to nitrocellulose membranes. The blotted polypeptides were probed with biotinylated Ricinus communis lectin (RCA120) followed by streptavidin/alkaline phosphatase. This procedure detected five asialoglycoproteins (a2-macroglobulin, transferrin, a1-antitrypsin, a1-antichymotrypsin and haptoglobin ß chain). The asialoform of the a1-trypsin inhibitor was found to be decreased in inflammation. |
Identificador | |
Idioma(s) |
eng |
Direitos |
info:eu-repo/semantics/restrictedAccess |
Fonte |
Lundy , F & Wisdom , G B 1992 , ' The determination of asialoglycoforms of serum glycoproteins by lectin blotting with Ricinus communis agglutinin ' Clinica Chimica Acta , vol 205 (3) , pp. 187-195 . DOI: 10.1016/0009-8981(92)90059-Y |
Tipo |
article |