Identification of a major GpVI-binding locus in human type III collagen
Data(s) |
15/05/2008
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Resumo |
We have analyzed the adhesion of human and murine platelets, and of recombinant human and murine GpVI ectodomains, to synthetic triple-helical collagen-like peptides. These included 57 peptides derived from the sequence of human type III collagen and 9 peptides derived from the cyanogen bromide fragment of bovine type III collagen, alpha 1(III)CB4. We have identified several peptides that interact with GpVI, in particular a peptide designated III-30 with the sequence GAOGLRGGAGPOG-PEGGKGAAGPOGPO. Both human and murine platelets bound to peptide III-30 in a GpVI-dependent manner. III-30 also supported binding of recombinant GpVI ectodomains. Cross-linked III-30 induced aggregation of human and murine platelets, although with a lower potency than collagen-related peptide. Modifications of the peptide sequence indicated that the hydroxyproline residues play a significant role in supporting its GpVI reactivity. However, many peptides containing OGP/ GPO motifs did not support adhesion to GpVI. These data indicate that the ability of a triple-helical peptide to bind GpVI is not solely determined by the presence or spatial arrangement of these OGP/GPO motifs within the peptides. |
Identificador | |
Idioma(s) |
eng |
Direitos |
info:eu-repo/semantics/restrictedAccess |
Fonte |
Jarvis , G , Raynal , N , Langford , J P , Onley , D J , Andrews , A , Smethurst , P A & Farndale , R W 2008 , ' Identification of a major GpVI-binding locus in human type III collagen ' Blood , vol 111 , no. 10 , pp. 4986-4996 . DOI: 10.1182/blood-2007-08-108472 |
Palavras-Chave | #/dk/atira/pure/subjectarea/asjc/1300/1303 #Biochemistry #/dk/atira/pure/subjectarea/asjc/1300/1307 #Cell Biology #/dk/atira/pure/subjectarea/asjc/2400/2403 #Immunology #/dk/atira/pure/subjectarea/asjc/2700/2720 #Hematology |
Tipo |
article |