Retinal VEGF mRNA measured by SYBR Green I fluorescence: A versatile approach to quantitative PCR
| Data(s) |
2000
|
|---|---|
| Identificador |
http://www.scopus.com/inward/record.url?scp=0034609681&partnerID=8YFLogxK |
| Idioma(s) |
eng |
| Direitos |
info:eu-repo/semantics/restrictedAccess |
| Fonte |
Simpson , D , Feeney , S , Boyle , C & Stitt , A 2000 , ' Retinal VEGF mRNA measured by SYBR Green I fluorescence: A versatile approach to quantitative PCR ' Molecular Vision , vol 6 , no. 24 , pp. 178-183 . |
| Palavras-Chave | #/dk/atira/pure/subjectarea/asjc/2700/2731 #Ophthalmology |
| Tipo |
article |
| Resumo |
PURPOSE: To determine whether continuous monitoring of SYBR Green I fluorescence provides a reliable and flexible method of quantitative RT-PCR. Our aims were (i) to test whether SYBR Green I analysis could quantify a wide range of known VEGF template concentrations, (ii) to apply this method in an experimental model, and (iii) to determine whether 20 existing primer pairs could be used to quantify their cognate mRNAs. |
