Inhibition of cathepsin L-like proteases by cathepsin V propeptide
Data(s) |
01/05/2007
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Resumo |
The N-terminal propeptide domains of several cathepsin L-like cysteine proteases have been shown to possess potent inhibitory activity. Here we report the first kinetic characterisation of the inhibition properties of the cathepsin V propeptide (CatV PP). Using a facile recombinant approach we demonstrate expression, purification and evaluation of the CatV PP. This propeptide was found to behave as a tight-binding inhibitor against CatV (K (i) 10.2 nm). It also functions as an inhibitor against other members of the CatL-like subclass (CatL, 9.8 nm; CatS, 10.7 nm; and CatK, 149 nm) and had no discernible effects upon the more distantly related CatB. |
Identificador |
http://dx.doi.org/10.1515/BC.2007.053 http://www.scopus.com/inward/record.url?scp=34249106050&partnerID=8YFLogxK |
Idioma(s) |
eng |
Direitos |
info:eu-repo/semantics/restrictedAccess |
Fonte |
Burden , R , Snoddy , P , Jefferies , C A , Walker , B & Scott , C 2007 , ' Inhibition of cathepsin L-like proteases by cathepsin V propeptide ' Biological Chemistry , vol 388(5) , no. 5 , pp. 541-545 . DOI: 10.1515/BC.2007.053 |
Palavras-Chave | #/dk/atira/pure/subjectarea/asjc/1300/1303 #Biochemistry |
Tipo |
article |