Dynamics and Function of Langerhans Cells In Vivo: Dermal Dendritic Cells Colonize Lymph Node Areas Distinct from Slower Migrating Langerhans Cells


Autoria(s): Kissenpfennig, Adrien; Henri, S.; Dubois, B.; Laplace-Builhe, C.; Perrin, P.; Romani, N.; Tripp, C.H.; Douillard, P.; Leserman, L.; Kaiserlian, D.; Saeland, S.; Davoust, J.; Malissen, B.
Data(s)

01/05/2005

Resumo

Langerhans cells (LCs) are prominent dendritic cells (DCs) in epithelia, but their role in immunity is poorly defined. To track and discriminate LCs from dermal DCs in vivo, we developed knockin mice expressing enhanced green fluorescent protein (EGFP) under the control of the langerin (CD207) gene. By using vital imaging, we showed that most EGFP(+) LCs were sessile under steady-state conditions, whereas skin inflammation induced LC motility and emigration to lymph nodes (LNs). After skin immunization, dermal DCs arrived in LNs first and colonized areas distinct from slower migrating LCs. LCs reaching LNs under steady-state or inflammatory conditions expressed similar levels of costimulatory molecules. Langerin and EGFP were also expressed on thymic DCs and on blood-derived, CD8alpha(+) DCs from all secondary lymphoid organs. By using a similar knockin strategy involving a diphtheria toxin receptor (DTR) fused to EGFP, we demonstrated that LCs were dispensable for triggering hapten-specific T cell effectors through skin immunization.

Formato

application/pdf

Identificador

http://pure.qub.ac.uk/portal/en/publications/dynamics-and-function-of-langerhans-cells-in-vivo-dermal-dendritic-cells-colonize-lymph-node-areas-distinct-from-slower-migrating-langerhans-cells(99a1eecd-aebd-4092-b4dd-2e796ff7d3eb).html

http://dx.doi.org/10.1016/j.immuni.2005.04.004

http://pure.qub.ac.uk/ws/files/3219320/immunity2005.pdf

http://www.scopus.com/inward/record.url?scp=21144444056&partnerID=8YFLogxK

Idioma(s)

eng

Direitos

info:eu-repo/semantics/openAccess

Fonte

Kissenpfennig , A , Henri , S , Dubois , B , Laplace-Builhe , C , Perrin , P , Romani , N , Tripp , C H , Douillard , P , Leserman , L , Kaiserlian , D , Saeland , S , Davoust , J & Malissen , B 2005 , ' Dynamics and Function of Langerhans Cells In Vivo: Dermal Dendritic Cells Colonize Lymph Node Areas Distinct from Slower Migrating Langerhans Cells ' Immunity , vol 22(5) , no. 5 , pp. 643-654 . DOI: 10.1016/j.immuni.2005.04.004

Palavras-Chave #/dk/atira/pure/subjectarea/asjc/2700/2723 #Immunology and Allergy #/dk/atira/pure/subjectarea/asjc/2700/2725 #Infectious Diseases #/dk/atira/pure/subjectarea/asjc/2400/2403 #Immunology
Tipo

article