Chlamydia trachomatis Infection Leads to Defined Alterations to the Lipid Droplet Proteome in Epithelial Cells.
Data(s) |
2015
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Identificador |
http://www.ncbi.nlm.nih.gov/pubmed/25909443 PONE-D-14-45569 PLoS One, 2015, 10 (4), pp. e0124630 - ? http://hdl.handle.net/10161/10589 1932-6203 |
Relação |
PLoS One 10.1371/journal.pone.0124630 |
Tipo |
Journal Article |
Cobertura |
United States |
Resumo |
The obligate intracellular bacterium Chlamydia trachomatis is a major human pathogen and a main cause of genital and ocular diseases. During its intracellular cycle, C. trachomatis replicates inside a membrane-bound vacuole termed an "inclusion". Acquisition of lipids (and other nutrients) from the host cell is a critical step in chlamydial replication. Lipid droplets (LD) are ubiquitous, ER-derived neutral lipid-rich storage organelles surrounded by a phospholipids monolayer and associated proteins. Previous studies have shown that LDs accumulate at the periphery of, and eventually translocate into, the chlamydial inclusion. These observations point out to Chlamydia-mediated manipulation of LDs in infected cells, which may impact the function and thereby the protein composition of these organelles. By means of a label-free quantitative mass spectrometry approach we found that the LD proteome is modified in the context of C. trachomatis infection. We determined that LDs isolated from C. trachomatis-infected cells were enriched in proteins related to lipid metabolism, biosynthesis and LD-specific functions. Interestingly, consistent with the observation that LDs intimately associate with the inclusion, a subset of inclusion membrane proteins co-purified with LD protein extracts. Finally, genetic ablation of LDs negatively affected generation of C. trachomatis infectious progeny, consistent with a role for LD biogenesis in optimal chlamydial growth. |
Formato |
e0124630 - ? |
Idioma(s) |
ENG |
Palavras-Chave | #Animals #Cell Line #Chlamydia Infections #Chlamydia trachomatis #Epithelial Cells #HeLa Cells #Humans #Lipid Droplets #Lipid Metabolism #Mice #Proteome #Proteomics |