Functional properties of cell-seeded three-dimensionally woven poly(epsilon-caprolactone) scaffolds for cartilage tissue engineering.
Data(s) |
01/04/2010
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Formato |
1291 - 1301 |
Identificador |
http://www.ncbi.nlm.nih.gov/pubmed/19903085 Tissue Eng Part A, 2010, 16 (4), pp. 1291 - 1301 http://hdl.handle.net/10161/3355 1937-335X |
Idioma(s) |
ENG en_US |
Relação |
Tissue Eng Part A 10.1089/ten.TEA.2009.0480 Tissue Engineering Part a |
Tipo |
Journal Article |
Cobertura |
United States |
Resumo |
Articular cartilage possesses complex mechanical properties that provide healthy joints the ability to bear repeated loads and maintain smooth articulating surfaces over an entire lifetime. In this study, we utilized a fiber-reinforced composite scaffold designed to mimic the anisotropic, nonlinear, and viscoelastic biomechanical characteristics of native cartilage as the basis for developing functional tissue-engineered constructs. Three-dimensionally woven poly(epsilon-caprolactone) (PCL) scaffolds were encapsulated with a fibrin hydrogel, seeded with human adipose-derived stem cells, and cultured for 28 days in chondrogenic culture conditions. Biomechanical testing showed that PCL-based constructs exhibited baseline compressive and shear properties similar to those of native cartilage and maintained these properties throughout the culture period, while supporting the synthesis of a collagen-rich extracellular matrix. Further, constructs displayed an equilibrium coefficient of friction similar to that of native articular cartilage (mu(eq) approximately 0.1-0.3) over the prescribed culture period. Our findings show that three-dimensionally woven PCL-fibrin composite scaffolds can be produced with cartilage-like mechanical properties, and that these engineered properties can be maintained in culture while seeded stem cells regenerate a new, functional tissue construct. |
Palavras-Chave | #Adult Stem Cells #Biocompatible Materials #Biomechanical Phenomena #Cartilage, Articular #Chondrocytes #Chondrogenesis #Collagen #Compressive Strength #Extracellular Matrix #Fibrin #Friction #Humans #Hydrogels #Materials Testing #Microscopy, Electron, Scanning #Polyesters #Regeneration #Shear Strength #Tensile Strength #Tissue Engineering #Tissue Scaffolds |