Identification of ribosomal proteins specific to higher eukaryotic organisms
Data(s) |
01/11/2002
|
---|---|
Resumo |
This report describes the identification of a novel protein named PS1D (Genbank accession number ), which is composed of an S1-like RNA-binding domain, a (cysteine)x3-(histidine) CCCH-zinc finger, and a very basic carboxyl domain. PS1D is expressed as two isoforms, probably resulting from the alternative splicing of mRNA. The long PS1D isoform differs from the short one by the presence of 48 additional amino acids at its amino-terminal extremity. Analysis of PS1D subcellular distribution by cell fractionation reveals that this protein belongs to the core of the eukaryotic 60S ribosomal subunit. Interestingly, PS1D protein is a highly conserved protein among mammalians as murine, human, and simian PS1D homologues share more than 95% identity. In contrast, no homologous protein is found in lower eukaryotes such as yeast and Caenorhabditis elegans. These observations indicate that PS1D is the first eukaryotic ribosomal protein that is specific to higher eukaryotes. Journal Article Research Support, Non-U.S. Gov't SCOPUS: ar.j info:eu-repo/semantics/published |
Formato |
1 full-text file(s): application/pdf |
Identificador |
uri/info:doi/10.1074/jbc.M208551200 uri/info:pii/M208551200 uri/info:pmid/12202495 https://dipot.ulb.ac.be/dspace/bitstream/2013/50952/1/45034.full.pdf http://hdl.handle.net/2013/ULB-DIPOT:oai:dipot.ulb.ac.be:2013/50952 |
Idioma(s) |
en |
Direitos |
1 full-text file(s): info:eu-repo/semantics/restrictedAccess |
Fonte |
The Journal of biological chemistry, 277 (47 |
Palavras-Chave | #Sciences bio-médicales et agricoles #Amino Acid Sequence #Animals #Cell Fractionation #Cell Line #Genes, Reporter #Humans #Mice #Molecular Sequence Data #Nuclear Proteins -- chemistry #Nuclear Proteins -- genetics #Nuclear Proteins -- isolation & purification #Nuclear Proteins -- metabolism #Protein Isoforms #RNA, Ribosomal -- genetics #RNA, Ribosomal -- metabolism #RNA-Binding Proteins -- chemistry #RNA-Binding Proteins -- genetics #RNA-Binding Proteins -- isolation & purification #RNA-Binding Proteins -- metabolism #Recombinant Fusion Proteins -- genetics #Recombinant Fusion Proteins -- metabolism #Ribosomal Proteins -- chemistry #Ribosomal Proteins -- genetics #Ribosomal Proteins -- isolation & purification #Ribosomal Proteins -- metabolism #Ribosomes -- chemistry #Ribosomes -- genetics #Ribosomes -- metabolism #Sequence Alignment #Tissue Distribution |
Tipo |
info:eu-repo/semantics/article info:ulb-repo/semantics/articlePeerReview info:ulb-repo/semantics/openurl/article |