Critical role of interleukin 5 and eosinophils in concanavalin A-induced hepatitis in mice.
Data(s) |
01/06/2002
|
---|---|
Resumo |
BACKGROUND & AIMS: Eosinophils are observed in several liver diseases, but their contribution in the pathogenesis of these disorders remains poorly investigated. Concanavalin A (Con A)-induced hepatitis is an experimental model of immune-mediated liver injury in which natural killer T (NKT) cells play a critical role through the production of interleukin (IL)-4 and the expression of Fas ligand (FasL). Because activated NKT cells also produce IL-5, a critical cytokine for eosinophil maturation and function, the role of IL-5 was investigated in this model. METHODS: IL-5-deficient mice, eosinophil depletion in wild-type (WT) mice, and NKT cell transfer from WT- or IL-5-deficient mice into NKT cell-deficient mice were used to assess the role of IL-5 and eosinophils. RESULTS: Liver eosinophil infiltrate and IL-5 production were observed after Con A challenge. Liver injury was dramatically reduced in IL-5-deficient or eosinophil-depleted mice. In addition, residual hepatitis observed in Fas-deficient mice was abolished after IL-5 neutralization. Finally, we showed that NKT cells constituted a critical source of IL-5. Indeed, transfer of WT NKT cells to mice lacking NKT cells restored liver injury, whereas transfer of IL-5-deficient NKT cells did not. CONCLUSIONS: These observations highlight the pathologic role of IL-5 and eosinophils in experimental immune-mediated hepatitis. Journal Article Research Support, Non-U.S. Gov't info:eu-repo/semantics/published |
Formato |
No full-text files |
Identificador |
uri/info:pii/S0016508502000331 uri/info:pmid/12055605 local/VX-005658 http://hdl.handle.net/2013/ULB-DIPOT:oai:dipot.ulb.ac.be:2013/50824 |
Idioma(s) |
en |
Fonte |
Gastroenterology, 122 (7 |
Palavras-Chave | #Sciences bio-médicales et agricoles #Animals #Antibodies, Monoclonal -- pharmacology #Antigens, CD95 -- genetics #Antigens, CD95 -- physiology #Concanavalin A #Drug-Induced Liver Injury -- pathology #Drug-Induced Liver Injury -- physiopathology #Eosinophils -- physiology #Interleukin-5 -- immunology #Interleukin-5 -- physiology #Killer Cells, Natural -- metabolism #Mice #Mice, Inbred C57BL #Mice, Knockout -- genetics |
Tipo |
info:eu-repo/semantics/article info:ulb-repo/semantics/articlePeerReview info:ulb-repo/semantics/openurl/article |