Electrochromatographic evaluation of a silica monolith capillary column for separation of basic pharmaceuticals
Data(s) |
01/02/2005
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Resumo |
A silica-based monolithic capillary column was prepared via a sol-gel process. The continuous skeleton and large through-pore structure were characterized by scanning electron microscopy (SEM). The native silica monolith has been successfully employed in the electrochromatographic separation of beta-blockers and alkaloids extracted from traditional Chinese medicines (TCMs). Column efficiencies greater than 250000 plates/m for capillary electrochromatography (CEC) separation of basic compounds were obtained. It was observed that retention of basic pharmaceuticals on the silica monolith was mainly contributed by a cation-exchange mechanism. Other retention mechanisms including reversed-phase and normal-phase mechanisms and electrophoresis of basic compounds also played a role in separation. A comparison of the differences between CEC and capillary zone electrophoresis (CZE) separation was also discussed. |
Identificador | |
Idioma(s) |
英语 |
Fonte |
谢传辉;胡继伟;肖华;苏兴业;董靖;田瑞军;何治柯;邹汉法.Electrochromatographic evaluation of a silica monolith capillary column for separation of basic pharmaceuticals,Electrophoresis,2005,26(4—5):790-797 |
Palavras-Chave | #basic pharmaceutical #electrochromatography #retention mechanism #silica monolith |
Tipo |
期刊论文 |