CE determination of 2-(9-carbazole)ethyl chloroformate-labeled oligopeptides
Data(s) |
2008
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Resumo |
A pre-column derivatization method for sensitive determination of oligopeptides, using the tagging reagent 2-(9-carbazole)ethyl chloroformate (CEOC-Cl) followed by capillary electrophoresis (CE) with diode-array detection, has been developed. Maximum yield close to 100% were observed when a three to fourfold molar excess of reagent was used at pH 9.0-10.0. Excess reagent was extracted with n-hexane-ethyl acetate 9:1-10:1 (v/v); this enabled direct analysis using CE with no significant disturbance from the major fluorescent reagent degradation by-products. The effects on the results of buffer pH and of SDS and organic modifier concentrations were examined. Good baseline resolution in the separation of five CEOC-peptides was achieved with a 48.5-cm total length (effective length 40 cm) 50-mu m inner diameter capillary column. |
Identificador | |
Idioma(s) |
英语 |
Fonte |
Ding Chenxu,Sun Xuejun,Zhao Xianen,Zhao Wenchen,Li Yulin, Wang Honglun,Suo Yourui,You Jinmao.CE determination of 2-(9-carbazole)ethyl chloroformate-labeled oligopeptides.CHROMATOGRAPHIA,2008,67(3-4):245-251 |
Palavras-Chave | #生物科学 #capillary electrophoresis #2-(9-carbazole)ethyl chloroformate (CEOC-Cl) #oligopeptides #derivatization |
Tipo |
期刊论文 |