Expression of TNF-alpha gene in Anabaena sp PCC 7120 and purification of its product by affinity chromatography
| Data(s) |
2001
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|---|---|
| Resumo |
The effects of N (NaNO3) and C (NaAc) source in medium on the expression of tumor necrosis factor-alpha (TNF-alpha) gene in transgenic Anabaena sp. PCC 7120 were compared. The data showed that N source stabilized the expression of foreign protein and C source altered the synthesis of cell walls. Comparing several methods for breaking the cells, supersonic was able to extract TNF-alpha better than others. For purification of TNF-alpha, transgenic Anabaena cells were broken, the extracts were precipitated with ammonia sulfate, and the impure TNF-alpha was eluted from DEAE ion exchange chromatography. Electrophoresis (PAGE-SDS) showed a single band at 17 kD position. The effects of N (NaNO3) and C (NaAc) source in medium on the expression of tumor necrosis factor-alpha (TNF-alpha) gene in transgenic Anabaena sp. PCC 7120 were compared. The data showed that N source stabilized the expression of foreign protein and C source altered the synthesis of cell walls. Comparing several methods for breaking the cells, supersonic was able to extract TNF-alpha better than others. For purification of TNF-alpha, transgenic Anabaena cells were broken, the extracts were precipitated with ammonia sulfate, and the impure TNF-alpha was eluted from DEAE ion exchange chromatography. Electrophoresis (PAGE-SDS) showed a single band at 17 kD position. |
| Identificador | |
| Idioma(s) |
英语 |
| Fonte |
Shi, DJ; Ye, X; Zhong, H; Cai, YY; Shao, N; Peng, GH; Dong, XJ; Wang, CM; Ouyang, YX; Li, ZJ.Expression of TNF-alpha gene in Anabaena sp PCC 7120 and purification of its product by affinity chromatography,ACTA BOTANICA SINICA,2001,43(1):46-50 |
| Palavras-Chave | #Biochemistry & Molecular Biology; Plant Sciences #tumor necrosis factor-alpha (TNF-alpha) #transgenic cyanobacterium (Anabaena sp PCC 7120) #affinity chromatography |
| Tipo |
期刊论文 |
