Foreign gene transfer into Chinese shrimps (Penaeus chinensis) with gene gun
Data(s) |
01/05/2001
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Resumo |
Plasmids pG DNA-RZ1 with a GFP (green fluorescent protein) reporter gene and a ribozyme gene incising penaeid white spot baculovirus (WSBV) were first introduced into the fertilized eggs of Chinese shrimps by gene gun. The treated and control samples of different development stages were observed with a fluorescent microscope. The transient expression of GFP gene was high in nauplius and zoea larvae. Results from RT-PCR and PCR for adults showed that the foreign genes had been transferred into the shrimps and had expressed the corresponding proteins. This work has established a transgenic method for penaeid shrimps, which will set base for the application of genetic engineering breeding into industry. Plasmids pG DNA-RZ1 with a GFP (green fluorescent protein) reporter gene and a ribozyme gene incising penaeid white spot baculovirus (WSBV) were first introduced into the fertilized eggs of Chinese shrimps by gene gun. The treated and control samples of different development stages were observed with a fluorescent microscope. The transient expression of GFP gene was high in nauplius and zoea larvae. Results from RT-PCR and PCR for adults showed that the foreign genes had been transferred into the shrimps and had expressed the corresponding proteins. This work has established a transgenic method for penaeid shrimps, which will set base for the application of genetic engineering breeding into industry. |
Identificador | |
Idioma(s) |
英语 |
Fonte |
Liu, ZY; Xiang, JH; Zhou, GY; Gong, ZX.Foreign gene transfer into Chinese shrimps (Penaeus chinensis) with gene gun,CHINESE SCIENCE BULLETIN,2001,46(9):766-770 |
Palavras-Chave | #Multidisciplinary Sciences #gene gun #GFP #transgenic shrimps |
Tipo |
期刊论文 |