Label free electrochemiluminescence protocol for sensitive DNA detection with a tris(2,2'-bipyridyl)ruthenium(II) modified electrode based on nucleic acid oxidation
Data(s) |
2007
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Resumo |
Label free electrochemiluminescence (ECL) DNA detection based on catalytic guanine and adenine bases oxidation using tris(2,2'-bipyridyl)ruthenium(II) [Ru(bpy)(3)(2+)] modified glassy carbon (GC) electrode was demonstrated in this work. The modified GC electrode was prepared by casting carbon nanotubes (CNT)/Nafion/Ru(bpy)(3)(2+) composite film on the electrode surface. ECL signals of doublestranded DNA and their thermally denatured counterparts can be distinctly discriminated using cyclic voltammetry (CV) with a low concentration (3.04 x 10(-8) mol/L for Salmon Testes-DNA). Most importantly, sensitive single-base mismatch detection of p53 gene sequence segment was realized with 3.93 x 10(-10) mol/L employing CV stimulation (ECL signal of C/A mismatched DNA oligonucleotides was 1.5-fold higher than that of fully base-paired DNA oligonucleotides). Label free, high sensitivity and simplicity for single-base mismatch discrimination were the main advantages of the present ECL technique for DNA detection over the traditional DNA sensors. |
Identificador | |
Idioma(s) |
英语 |
Fonte |
Wei H;Du Y;Kang JZ;Wang EK.Label free electrochemiluminescence protocol for sensitive DNA detection with a tris(2,2'-bipyridyl)ruthenium(II) modified electrode based on nucleic acid oxidation,ELECTROCHEMISTRY COMMUNICATIONS,2007 ,9(7):1474-1479 |
Palavras-Chave | #ELECTROGENERATED CHEMILUMINESCENCE #GUANINE OXIDATION #ULTRATHIN FILMS #RU(BPY)(3)(2+) #RUTHENIUM(II) #NANOPARTICLES #HYBRIDIZATION #ISOTOPE #SOLVENT #TARGETS |
Tipo |
期刊论文 |