Quantitative electrochemiluminescence detection of proteins: Avidin-based sensor and tris(2,2 '-bipyridine) ruthenium(II) label
Data(s) |
2008
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Resumo |
Quantitative electrochemilumineseence (ECL) detection of a model protein, bovine serum albumin (BSA) was achieved via biotin-avidin interaction using an avidin-based sensor and a well-developed ECL system of tris(2,2'-bipyridine) ruthenium(II) derivative as label and tri-n-propylamine (TPA) as coreactant. To detect the protein, avidin was linked to the glassy carbon electrode through passive adsorptions and covalent interaction with carboxylate-terminated carbon nanotubes that was used as binder to immobilize avidin onto the electrode. Then, biotinylated BSA tagged with tris(2,2'-bipyridine) ruthenium(II) label was attached to the prepared avidin surface. |
Identificador | |
Idioma(s) |
英语 |
Fonte |
Fang LY;Lu ZZ;Wei H;Wang EK.Quantitative electrochemiluminescence detection of proteins: Avidin-based sensor and tris(2,2 '-bipyridine) ruthenium(II) label,BIOSENSORS & BIOELECTRONICS,2008,23(11 ):1645-1651 |
Palavras-Chave | #C-REACTIVE PROTEIN #ELECTROGENERATED CHEMILUMINESCENCE #CARBON NANOTUBES #CAPILLARY-ELECTROPHORESIS #IMPEDANCE SPECTROSCOPY #HIGH AMPLIFICATION #ELECTRODES #DNA #ASSEMBLIES #COMPLEXES |
Tipo |
期刊论文 |