Rapid detection of porcine circovirus type 2 by loop-mediated isothermal amplification
| Data(s) |
2008
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| Resumo |
A method of loop-mediated isothermal amplification (LAMP) was employed to develop a rapid and simple detection system for porcine circovirus type 2 (PCV2). The amplification could be finished in 60 min under isothermal condition at 64 degrees C by employing a set of four primers targeting the cap gene of PCV2. The LAMP assay showed higher sensitivity than the conventional PCR, with a detection limit of five copies per tube of purified PCV2 genomic DNA. No cross-reactivity was observed from the samples of other related viruses including porcine circovirus type 1 (PCV1), porcine parvovirus (PPV), porcine pseudorabies virus (PRV) and porcine reproductive and respiratory syndrome virus (PRRSV). The detection rate of PCV2 LAMP for 86 clinical samples was 96.5% and appeared greater than that of the PCR method. The LAMP assay reported can provide a rapid yet simple test of PCV2 suitable for laboratory diagnosis and pen-side detection due to ease of operation and the requirement of only a regular water bath or heat block for the reaction. (c) 2008 Elsevier B.V. All rights reserved. |
| Identificador | |
| Idioma(s) |
英语 |
| Fonte |
Chen, HT (Chen, Hao-tai); Zhang, J (Zhang, Jie); Sun, DH (Sun, De-hui); Chu, YF (Chu, Yue-feng); Cai, XP (Cai, Xue-peng); Liu, XT (Liu, Xiang-tao); Luo, XN (Luo, Xue-nong); Liu, Q (Liu, Qing); Liu, YS (Liu, Yong-sheng). Rapid detection of porcine circovirus type 2 by loop-mediated isothermal amplification , JOURNAL OF VIROLOGICAL METHODS,2008,149(2):264-268 |
| Palavras-Chave | #porcine circovirus type 2 (PCV2) #detection #loop-mediated isothermal amplification (LAMP) #sensitivity #specificity |
| Tipo |
期刊论文 |
