Multiplex detection of mutations in clinical isolates of rifampin-resistant Mycobacterium tuberculosis by short oligonucleotide Ligation assay on DNA chips
Data(s) |
2004
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Resumo |
A new approach, short-oligonucleotide-ligation assay on DNA chip (SOLAC), is developed to detect mutations in rifampin-resistant Mycobacterium tuberculosis. The method needs only four common probes to detect 15 mutational variants of the rpoB gene within 12 h. Fifty-five rifampin-resistant M. tuberculosis isolates were analyzed, resulting in 87.3% accuracy and 83.6% concordance relative to DNA sequencing. |
Identificador | |
Idioma(s) |
英语 |
Fonte |
Jiao-Yu Deng; Xian-En Zhang; Hong-Bing Lu; Qiang Liu; Zhi-Ping Zhang; Ya-Feng Zhou; Wei-Hong Xie and Zhi-Jun Fu.Multiplex detection of mutations in clinical isolates of rifampin-resistant Mycobacterium tuberculosis by short oligonucleotide Ligation assay on DNA chips,JOURNAL OF CLINICAL MICROBIOLOGY,2004,42(10):4850-4852 |
Palavras-Chave | #Microbiology #POLYMERASE-CHAIN-REACTION #LINE PROBE ASSAY #RAPID DETECTION #DRUG-RESISTANCE #RPOB MUTATIONS #GENE #STRAINS |
Tipo |
期刊论文 |