The survey on determination of malathion biomarker with genotoxicity and ecophysiological reactions in the Caspian roach (Rutilus rutilus caspicus)


Autoria(s): Farokhi, Farzaneh
Data(s)

2014

Resumo

In the present study possibility of Malathion biomarker with Genotoxicity and Ecophysiological reactions were determined in Caspian Roach (Rutilus rutilus caspicus). At fist LC50 value of Malathion, an organophosphate insecticide was determined. Then four groups of experimental fish (containing 30 fish in each group) were exposed to different concentrations of Malathion. e. 0, 0.01, 0.05 and 0/1 ppm respectively for 23 days and effects of Malathion on Hematological (RBC, WBC, Hb and Hct) and biochemical parameters (Glucose, triglyceride, urea, total protein and Albumin), some enzymes (SGPT, SGOT and ALP), Cortisol level, plasma cations (Na+ and K+) , histological changes (gill and liver) and finally DNA destruction were examined. Sampling was done in 3rd, 13th, 23rd days during exposure and also 30 days after recovery. Data analysis was done by SPSS (Ver.13) and graphs were drawn by Excel 2007. Results showed that WBC, RBC, Hb, Hct, some biochemical parameters and K+ of Mallation treatments were decreased significantly in compare to control group (P<0.05). Changes in enzyme were many different. No significant changes were observed in Na+ and cortisol levels (except in groups treated with 0.01 Mallation) (P>0.05). LC50 value of Malathion in Caspian Roach was 6.5 ppm. Histological examinations showed that Mallation cause tissue damages and there were more damages in longer times and in higher concentrations. Apoptotic cell and comet were observed as DNA destruction and they were more in treatments with higher Mallation concentrations for longer times.

Formato

application/pdf

Identificador

http://aquaticcommons.org/18984/1/42976.pdf

Farokhi, Farzaneh (2014) The survey on determination of malathion biomarker with genotoxicity and ecophysiological reactions in the Caspian roach (Rutilus rutilus caspicus). PhD Thesis, Islamic Azad University, Science and Research Branch, Tehran, 129pp.

Idioma(s)

fa

Relação

http://aquaticcommons.org/18984/

Palavras-Chave #Biology #Fisheries #Pollution
Tipo

Thesis

NonPeerReviewed