Cleavage of DNA by polyamide-seco-CBI conjugates
Data(s) |
2001
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Resumo |
Small molecules that bind to any predetermined DNA sequence in the human genome are potentially useful tools for molecular biology and human medicine. Polyamides containing N-methylimidazole (Im) N-methylpyrrole (Py) are cell permeable small molecules that bind DNA according to a set of "pairing rules" with affinities and specificities similar to many naturally occurring DNA binding proteins. Py-Im polyamides offer a general approach to the chemical regulation of gene expression. We demonstrate here that polyamide containing a DNA alkylating moiety seco-CBI can specifically direct sequence specific DNA alkylation. We can also control the strand of DNA that is alkylated, depending on the enantiomer of seco-CBI used and the orientation of the polyamide relative to the alkylation site (Chapter 2). This class of molecules has been applied to a gene repair system in collaboration with the Baltimore group at Caltech (Chapter 3). Also reported are additional seco-CBI polyamide conjugates synthesized to study other systems (HIV-1 and COX-2) (Appendix 1). |
Formato |
application/pdf |
Identificador |
http://thesis.library.caltech.edu/8137/1/Chang%202001.pdf Chang, Aileen Yulin (2001) Cleavage of DNA by polyamide-seco-CBI conjugates. Dissertation (Ph.D.), California Institute of Technology. http://resolver.caltech.edu/CaltechTHESIS:03142014-154815404 <http://resolver.caltech.edu/CaltechTHESIS:03142014-154815404> |
Relação |
http://resolver.caltech.edu/CaltechTHESIS:03142014-154815404 http://thesis.library.caltech.edu/8137/ |
Tipo |
Thesis NonPeerReviewed |