Surface-Bound Selectin-Ligand Binding Is Regulated By Carrier Diffusion
Data(s) |
2009
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Resumo |
Two-dimensional (2D) kinetics of receptor-ligand interactions governs cell adhesion in many biological processes. While the dissociation kinetics of receptor-ligand bond is extensively investigated, the association kinetics has much less been quantified. Recently receptor-ligand interactions between two surfaces were investigated using a thermal fluctuation assay upon biomembrane force probe technique (Chen et al. in Biophys J 94:694-701, 2008). The regulating factors on association kinetics, however, are not well characterized. Here we developed an alternative thermal fluctuation assay using optical trap technique, which enables to visualize consecutive binding-unbinding transition and to quantify the impact of microbead diffusion on receptor-ligand binding. Three selectin constructs (sLs, sPs, and PLE) and their ligand P-selectin glycoprotein ligand 1 were used to conduct the measurements. It was indicated that bond formation was reduced by enhancing the diffusivity of selectin-coupled carrier, suggesting that carrier diffusion is crucial to determine receptor-ligand binding. It was also found that 2D forward rate predicted upon first-order kinetics was in the order of sPs > sLs > PLE and bond formation was history-dependent. These results further the understandings in regulating association kinetics of surface-bound receptor-ligand interactions. |
Identificador | |
Idioma(s) |
英语 |
Fonte |
European Biophysics Journal With Biophysics Letters, 2009, 38(5): 701-711 |
Palavras-Chave | #Association Kinetics #Regulating Factors #Carrier Diffusion #Optical Trap #Selectin #Ligand #Mediated Cell-Adhesion #P-Selectin #Glycoprotein Ligand-1 #Optical Tweezers #Shear-Flow #Human Neutrophils #Force Microscopy #Association Rate #Contact Area #Kinetics |
Tipo |
期刊论文 |