Soluble Angiopoietin Receptor Tie-2 In Patients With Acute Myocardial Infarction And Its Detection By Optical Protein-Chip


Autoria(s): Wang CL; Li JQ; Li HW; Jin G(靳刚); Wang ZH(王战会); Meng YH(孟永宏); Xiu RJ
Data(s)

2009

Resumo

The Tie-2 receptor has been shown to play a role in angiogenesis in atherosclerosis. The conventional method assaying the level of soluble Tie-2 (sTie-2) was ELISA. However, this method has some disadvantages. The aims of this research are to establish a more simple detection method, the optical protein-chip based on imaging ellipsomtry (OPC-IE) applying to Tie-2 assay. The sTie-2 biosensor surface on silicon wafer was prepared first, and then serum levels of sTie-2 in 38 patients with AMI were measured on admission (day 1), day 2, day 3 and day 7 after onset of chest pain and 41 healthy controls by ELISA and OPC-IE in parallel. Median level of sTie-2 increased significantly in the AMI patients when compared with the controls. Statistics showed there was a significant correlation in sTie-2 results between the two methods (r=0.923, P0.01). The result of this study showed that the level of sTie-2 increased in AMI, and OPC-IE assay was a fast, reliable, and convenient technique to measure sTie-2 in serum.

Identificador

http://dspace.imech.ac.cn/handle/311007/26690

http://www.irgrid.ac.cn/handle/1471x/9024

Idioma(s)

英语

Fonte

Artificial Cells Blood Substitutes And Biotechnology, 2009, 37(4): 183-186

Palavras-Chave #Soluble Tie-2 #Acute Myocardial Infarction #Elisa #Optical Protein-Chip #Imaging Ellipsometry #Imaging Ellipsometry #Tyrosine Kinase #Tek
Tipo

期刊论文