Molecular Techniques for Dicistrovirus Detection without RNA Extraction or Purification
Data(s) |
05/02/2014
05/02/2014
2013
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Resumo |
Dicistroviridae is a new family of small, nonenveloped, and +ssRNA viruses pathogenic to both beneficial arthropods and insect pests as well. Triatoma virus (TrV), a dicistrovirus, is a pathogen of Triatoma infestans (Hemiptera: Reduviidae), one of the main vectors of Chagas disease. In this work, we report a single-step method to identify TrV, a dicistrovirus, isolated from fecal samples of triatomines. The identification method proved to be quite sensitive, even without the extraction and purification of RNA virus. |
Identificador |
BioMed Research International 2013 : (2013) // Article ID 218593 2314-6133 http://hdl.handle.net/10810/11360 10.1155/2013/218593 |
Idioma(s) |
eng |
Publicador |
Hindawi Publishing Corporation |
Relação |
http://www.hindawi.com/journals/bmri/2013/218593/ |
Direitos |
© 2013 Jailson F. B. Querido et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. info:eu-repo/semantics/openAccess |
Palavras-Chave | #Triatoma virus #infestans hemiptera #reduviidae |
Tipo |
info:eu-repo/semantics/article |