Manual for starch gel electrophoresis: A method for the detection of genetic variation


Autoria(s): Aebersold, Paul B.; Winans, Gary A.; Teel, David J.; Milner, George B.; Utter, Fred M.
Data(s)

1987

Resumo

The procedure to conduct horizontal starch gel electrophoresis on enzymes is described in detail. Areas covered are (I) collection and storage of specimens, (2) preparation of tissues, (3) preparation of a starch gel, (4) application of enzyme extracts to a gel, (5) setting up a gel for electrophoresis, (6) slicing a gel, and (7) staining a gel. Recipes are also included for 47 enzyme stains and 3 selected gel buffers. (PDF file contains 26 pages.)

Formato

application/pdf

Identificador

http://aquaticcommons.org/2760/1/tr61.pdf

Aebersold, Paul B. and Winans, Gary A. and Teel, David J. and Milner, George B. and Utter, Fred M. (1987) Manual for starch gel electrophoresis: A method for the detection of genetic variation. NOAA/National Marine Fisheries Service, (NOAA Technical Report NMFS, 61)

Idioma(s)

en

Publicador

NOAA/National Marine Fisheries Service

Relação

http://aquaticcommons.org/2760/

http://spo.nwr.noaa.gov/tr61.pdf

Palavras-Chave #Ecology #Fisheries #Biology
Tipo

Monograph or Serial Issue

NonPeerReviewed