SNP Discovery in European Anchovy (Engraulis encrasicolus, L) by High-Throughput Transcriptome and Genome Sequencing


Autoria(s): Montes Asperilla, Iratxe; Conklin, Darrell; Albina Vivanco, Aitor; Creer, Simón; Carvalho, Gary R.; Santos, María; Estomba Recalde, Miren Andone
Data(s)

16/09/2013

16/09/2013

2013

Resumo

12 p.

Increased throughput in sequencing technologies has facilitated the acquisition of detailed genomic information in non-model species. The focus of this research was to discover and validate SNPs derived from the European anchovy (Engraulis encrasicolus) transcriptome, a species with no available reference genome, using next generation sequencing technologies. A cDNA library was constructed from four tissues of ten fish individuals corresponding to three populations of E. encrasicolus, and Roche 454 GS FLX Titanium sequencing yielded 19,367 contigs. Additionally, the European anchovy genome was sequenced for the same ten individuals using an Illumina HiSeq2000. Using a computational pipeline for combining transcriptome and genome information, a total of 18,994 SNPs met the necessary minor allele frequency and depth filters. A series of further stringent filters were applied to identify those SNPs likely to succeed in genotyping assays, and for filtering of those in potential duplicated genome regions. A novel method for detecting potential intron-exon boundaries in areas of putative SNPs has also been applied in silico to improve genotyping success. In all, 2,317 filtered putative transcriptome SNPs suitable for genotyping primer design were identified. From those, a subset of 530 were selected, with the genotyping results showing the highest reported conversion and validation rates (91.3% and 83.2%, respectively) reported to date for a non-model species. This study represents a promising strategy to discover genotypable SNPs in the exome of non-model organisms. The genomic resource generated for E. encrasicolus, both in terms of sequences and novel markers, will be informative for research into this species with applications including traceability studies, population genetic analyses and aquaculture.

Identificador

PLoS ONE 8(8) : (2013)

1932-6203

http://hdl.handle.net/10810/10572

10.1371/journal.pone.0070051

Idioma(s)

eng

Publicador

Public Library of Science

Relação

http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0070051

Direitos

© 2013 Montes et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

info:eu-repo/semantics/openAccess

Tipo

info:eu-repo/semantics/article