Plasmodium berghei glycine cleavage system T-protein is non-essential for parasite survival in vertebrate and invertebrate hosts
Data(s) |
2014
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Resumo |
T-protein, an aminomethyltransferase, represents one of the four components of glycine cleavage system (GCS) and catalyzes the transfer of methylene group from H-protein intermediate to tetrahydrofolate (THF) forming N-5, N-10-methylene THF (CH2-THF) with the release of ammonia. The malaria parasite genome encodes T-, H- and L-proteins, but not P-protein which is a glycine decarboxylase generating the aminomethylene group. A putative GCS has been considered to be functional in the parasite mitochondrion despite the absence of a detectable P-protein homologue. In the present study, the mitochondrial localization of T-protein in the malaria parasite was confirmed by immunofluorescence and its essentiality in the entire parasite life cycle was studied by targeting the T-protein locus in Plasmodium berghei (Pb). PbT knock out parasites did not show any growth defect in asexual, sexual and liver stages indicating that the T-protein is dispensable for parasite survival in vertebrate and invertebrate hosts. The absence of P-protein homologue and the non-essentiality of T protein suggest the possible redundancy of GCS activity in the malaria parasite. Nevertheless, the H- and L-proteins of GCS could be essential for malaria parasite because of their involvement in alpha-lcetoacid dehydrogenase reactions. (C) 2014 Elsevier B.V. All rights reserved. |
Formato |
application/pdf |
Identificador |
http://eprints.iisc.ernet.in/50788/1/mol_bio_par_197-1_50_2014.pdf Varadarajan, Nandan Mysore and Sundaram, Balamurugan and Subramani, Pradeep Annamalai and Kalappa, Devaiah Monnanda and Ghosh, Susanta Kumar and Nagaraj, Viswanathan Arun (2014) Plasmodium berghei glycine cleavage system T-protein is non-essential for parasite survival in vertebrate and invertebrate hosts. In: MOLECULAR AND BIOCHEMICAL PARASITOLOGY, 197 (1-2). pp. 50-55. |
Publicador |
ELSEVIER SCIENCE BV |
Relação |
http://dx.doi.org/ 10.1016/j.molbiopara.2014.10.003 http://eprints.iisc.ernet.in/50788/ |
Palavras-Chave | #Biochemistry #Centre for Infectious Disease Research and Biosafety Laboratories |
Tipo |
Journal Article PeerReviewed |