Characterization of a dual-active enzyme, DcpA, involved in cyclic diguanosine monophosphate turnover in Mycobacterium smegmatis


Autoria(s): Sharma, Indra Mani; Prakash, Sunita; Dhanaraman, Thillaivillalan; Chatterji, Dipankar
Data(s)

2014

Resumo

We have reported previously that the long-term survival of Mycobacterium smegmatis is facilitated by a dual-active enzyme MSDGC-1 (renamed DcpA), which controls the cellular turnover of cyclic diguanosine monophosphate (c-di-GMP). Most mycobacterial species possess at least a single copy of a DcpA orthologue that is highly conserved in terms of sequence similarity and domain architecture. Here, we show that DcpA exists in monomeric and dimeric forms. The dimerization of DcpA is due to non-covalent interactions between two protomers that are arranged in a parallel orientation. The dimer shows both synthesis and hydrolysis activities, whereas the monomer shows only hydrolysis activity. In addition, we have shown that DcpA is associated with the cytoplasmic membrane and exhibits heterogeneous cellular localization with a predominance at the cell poles. Finally, we have also shown that DcpA is involved in the change in cell length and colony morphology of M. smegmatis. Taken together, our study provides additional evidence about the role of the bifunctional protein involved in c-di-GMP signalling in M. smegmatis.

Formato

application/pdf

Identificador

http://eprints.iisc.ernet.in/50590/1/mic_sgm_160_2304_2014.pdf

Sharma, Indra Mani and Prakash, Sunita and Dhanaraman, Thillaivillalan and Chatterji, Dipankar (2014) Characterization of a dual-active enzyme, DcpA, involved in cyclic diguanosine monophosphate turnover in Mycobacterium smegmatis. In: MICROBIOLOGY-SGM, 160 (10). pp. 2304-2318.

Publicador

SOC GENERAL MICROBIOLOGY

Relação

http://dx.doi.org/ 10.1099/mic.0.080200-0

http://eprints.iisc.ernet.in/50590/

Palavras-Chave #Molecular Biophysics Unit
Tipo

Journal Article

PeerReviewed