A new pentameric structure of rotavirus NSP4 revealed by molecular replacement
Data(s) |
01/01/2012
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Resumo |
The region spanning residues 95-146 of the rotavirus nonstructural protein NSP4 from the asymptomatic human strain ST3 has been purified and crystallized and diffraction data have been collected to a resolution of 2.6 angstrom. Several attempts to solve the structure by the molecular-replacement method using the available tetrameric structures of this domain were unsuccessful despite a sequence identity of 73% to the already known structures. A more systematic approach with a dimer as the search model led to an unexpected pentameric structure using the program Phaser. The various steps involved in arriving at this molecular-replacement solution, which unravelled a case of subtle variation between different oligomeric states unknown at the time of solving the structure, are presented in this paper. |
Formato |
application/pdf |
Identificador |
http://eprints.iisc.ernet.in/43136/1/A_new_pentameric.pdf Chacko, Anita R and Jeyakanthan, J and Ueno, G and Sekar, K and Rao, Durga C and Dodson, Eleanor J and Suguna, Kaza and Read, Randy J (2012) A new pentameric structure of rotavirus NSP4 revealed by molecular replacement. In: Acta Crystallographica Section D, 68 (Part 1). pp. 57-61. |
Publicador |
International Union of Crystallography |
Relação |
http://scripts.iucr.org/cgi-bin/paper?S0907444911049705 http://eprints.iisc.ernet.in/43136/ |
Palavras-Chave | #Molecular Biophysics Unit |
Tipo |
Journal Article PeerReviewed |