Isolation of transforming growth factor-beta 2 cDNA from a fish, Cyprinus carpio by RT-PCR
Data(s) |
20/05/1997
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Resumo |
Transforming Growth Factors-beta (TGF-beta s) have been described in many vertebrate species of amphibians, aves and mammals. In this report we demonstrate the presence of TGF-beta 2 in pisces. TGF-beta 2 has been cloned from a fish, Cyrinus carpio, by RT-PCR using degenerate oligonucleotide primers. Sequence analysis of the amplified product and alignment of the deduced amino acid sequence with the human TGF-beta 2 amino acid sequence revealed 81% and 93% identity in the precursor and the mature regions, respectively. The northern blot analysis of fish heart RNA shows a major messenger RNA species of about 8.0 kb and two messages of very low abundance of about 5.0 kb and 4.0 kb. The identification of TGF-beta 2 isoform in Pisces and it's high degree of homology with the mammalian isoform suggests that among all TGF-beta isoforms, TGF-beta 2 is the most conserved during evolution. (C) 1997 Elsevier Science B.V. |
Formato |
application/pdf |
Identificador |
http://eprints.iisc.ernet.in/38280/1/Isolation_of_Transforming.pdf Sumathy, K and Desai, Kartiki V and Kondaiah, Paturu (1997) Isolation of transforming growth factor-beta 2 cDNA from a fish, Cyprinus carpio by RT-PCR. In: Gene, 191 (1). pp. 103-107. |
Publicador |
Elsevier Science |
Relação |
http://dx.doi.org/10.1016/S0378-1119(97)00048-6 http://eprints.iisc.ernet.in/38280/ |
Palavras-Chave | #Molecular Reproduction, Development & Genetics (formed by the merger of DBGL and CRBME) |
Tipo |
Journal Article PeerReviewed |