Crystallization and preliminary X-ray diffraction analysis of a galactose-specific lectin from the seeds of Butea monosperma


Autoria(s): Abhilash, Joseph; Geethanandan, K; Bharath, SR; Sadasivan, C; Haridas, M
Data(s)

01/04/2011

Resumo

The galactose-specific lectin from the seeds of Butea monosperma has been crystallized by the hanging-drop vapour-diffusion technique. The crystals belonged to space group P1, with unit-cell parameters a = 78.45, b = 78.91, c = 101.85 A, alpha = 74.30, beta = 76.65, gamma = 86.88 degrees. X-ray diffraction data were collected to a resolution of 2.44 A under cryoconditions (100 K) using a MAR image-plate detector system mounted on a rotating-anode X-ray generator. Molecular-replacement calculations carried out using the coordinates of several structures of legume lectins as search models indicate that the galactose-specific lectin from B. monosperma forms an octamer.

Formato

application/pdf

Identificador

http://eprints.iisc.ernet.in/37584/1/Crystallization.pdf

Abhilash, Joseph and Geethanandan, K and Bharath, SR and Sadasivan, C and Haridas, M (2011) Crystallization and preliminary X-ray diffraction analysis of a galactose-specific lectin from the seeds of Butea monosperma. In: Acta Crystallographica Section F, 67 (Part 4). pp. 524-526.

Publicador

John Wiley and Sons

Relação

http://scripts.iucr.org/cgi-bin/paper?S1744309111006853

http://eprints.iisc.ernet.in/37584/

Palavras-Chave #Molecular Biophysics Unit
Tipo

Journal Article

PeerReviewed