Maximum-entropy calculation of the electron density at 4 A resolution of Pf1 filamentous bacteriophage
Data(s) |
1983
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Resumo |
A 4 A electron-density map of Pf1 filamentous bacterial virus has been calculated from x-ray fiber diffraction data by using the maximum-entropy method. This method produces a map that is free of features due to noise in the data and enables incomplete isomorphous-derivative phase information to be supplemented by information about the nature of the solution. The map shows gently curved (banana-shaped) rods of density about 70 A long, oriented roughly parallel to the virion axis but slewing by about 1/6th turn while running from a radius of 28 A to one of 13 A. Within these rods, there is a helical periodicity with a pitch of 5 to 6 A. We interpret these rods to be the helical subunits of the virion. The position of strongly diffracted intensity on the x-ray fiber pattern shows that the basic helix of the virion is right handed and that neighboring nearly parallel protein helices cross one another in an unusual negative sense. |
Formato |
application/pdf |
Identificador |
http://eprints.iisc.ernet.in/35756/1/4728.full.pdf Bryan, RK and Bansal, Manju and Folkhard, , W and Nave, C and Marvin , DA (1983) Maximum-entropy calculation of the electron density at 4 A resolution of Pf1 filamentous bacteriophage. In: Proceedings Of The National Academy Of Sciences of the United States of America-Biological Sciences , 80 (15). 4728-4731 . |
Publicador |
National Academy of Sciences |
Relação |
http://www.pnas.org/content/80/15/4728.abstract http://eprints.iisc.ernet.in/35756/ |
Palavras-Chave | #Molecular Biophysics Unit |
Tipo |
Journal Article PeerReviewed |