Three-dimensional organization of fenestrae labyrinths in liver sinusoidal endothelial cells
Data(s) |
01/04/2009
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Resumo |
- Background/Aims Liver sinusoidal endothelial cell (LSEC) fenestrae are membrane-bound pores that are grouped in sieve plates and act as a bidirectional guardian in regulating transendothelial liver transport. The high permeability of the endothelial lining is explained by the presence of fenestrae and by various membrane-bound transport vesicles. The question as to whether fenestrae relate to other transport compartments remains unclear and has been debated since their discovery almost 40 years ago. - Methods In this study, novel insights concerning the three-dimensional (3D) organization of the fenestrated cytoplasm were built on transmission electron tomographical observations on isolated and cultured whole-mount LSECs. Classical transmission electron microscopy and atomic force microscopy imaging was performed to accumulate cross-correlative structural evidence. - Results and Conclusions The data presented here indicate that different arrangements of fenestrae have to be considered: i.e. open fenestrae that lack any structural obstruction mainly located in the thin peripheral cytoplasm and complexes of multifolded fenestrae organized as labyrinth-like structures that are found in the proximity of the perinuclear area. Fenestrae in labyrinths constitute about one-third of the total LSEC porosity. The 3D reconstructions also revealed that coated pits and small membrane-bound vesicles are exclusively interspersed in the non-fenestrated cytoplasmic arms. |
Identificador | |
Publicador |
John Wiley & Sons |
Relação |
DOI:10.1111/j.1478-3231.2008.01836.x Braet, Filip, Riches, James, Geerts, Willie, Jahn, Kristina A., Wisse, Eddie, & Frederik, Peter (2009) Three-dimensional organization of fenestrae labyrinths in liver sinusoidal endothelial cells. Liver International, 29(4), pp. 603-613. |
Fonte |
School of Earth, Environmental & Biological Sciences; Institute for Future Environments; Science & Engineering Faculty |
Palavras-Chave | #atomic force microscopy;electron tomography;fenestrae labyrinth;fenestrations;hepatic endothelium;liver sieve;liver sinusoidal endothelium;pores;porosity;TEM sections;transendothelial transport |
Tipo |
Journal Article |