Probiotics modify tight junction proteins in an animal model of non-alcoholic fatty liver disease
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24/03/2016
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Resumo |
Objectives We have investigated the effects of a multi–species probiotic preparation containing a combination of probiotic bacterial genera that included Bifidobacteria, Lactobacilli and a Streptococcus in a mouse model of high fat diet/obesity induced liver steatosis. Methods Three groups of C57B1/6J mice were fed either a standard chow or a high fat diet for 20 weeks, while a third group was fed a high fat diet for 10 weeks and then concomitantly administered probiotics for a further 10 weeks. Serum, liver and large bowel samples were collected for analysis. Results The expression of the tight junction proteins ZO-1 and ZO-2 was reduced (p < 0.05) in high fat diet fed mice compared to chow fed mice. Probiotic supplementation helped to maintain tight ZO-1 and ZO-2 expression compared with the high fat diet group (p < 0.05), but did not restore ZO-1 or ZO-2 expression compared with chow fed mice. Mice fed a high fat diet ± probiotics had significant steatosis development compared to chow fed mice (p < 0.05); steatosis was less severe in the probiotics group compared to the high fat diet group. Hepatic triglycerides concentration was higher in mice fed a high fat diet ± probiotics compared to the chow group (p < 0.05), and was lower in the probiotics group compared to the high fat diet group (p < 0.05). Compared to chow fed mice, serum glucose and cholesterol concentrations, and the activity of alanine transaminase were higher (p < 0.05), whereas serum triglyceride concentration was lower (p < 0.05) in mice fed a high fat diet ± probiotics. Conclusions Supplementation with a multi-species probiotic formulation helped to maintain tight junction proteins ZO-1 and ZO-2, and reduced hepatic triglyceride concentrations compared with a HFD alone. |
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application/pdf |
Identificador | |
Publicador |
SAGE Publications |
Relação |
http://eprints.qut.edu.au/94458/1/PROBIOTICS%20MODIFY%20TIGHT%20JUNCTION%20PROTEINS%20IN%20AN%20ANIMAL%20MODEL%20OF%20NON-ALCOHOLIC%20FATTY%20LIVER%20DISEASE.pdf https://au.sagepub.com/en-gb/oce/journal/therapeutic-advances-gastroenterology Briskey, David, Heritage, Mandy, Jaskowski, Lesley-Anne, Peake, Jonathan, Gobe, Glenda, Subramaniam, Nathan, Crawford, Darrell, Campbell, Catherine, & Vitetta, Luis (2016) Probiotics modify tight junction proteins in an animal model of non-alcoholic fatty liver disease. Therapeutic Advances in Gastroenterology. (In Press) http://purl.org/au-research/grants/NHMRC/1029574 |
Direitos |
Copyright 2016 SAGE Publications |
Fonte |
School of Biomedical Sciences; Faculty of Health; Institute of Health and Biomedical Innovation |
Palavras-Chave | #probiotics #nutrition #non-alcoholic fatty liver disease #metabolism #inflammation |
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Journal Article |