Successful recovery of preimplantation embryos by nonsurgical uterine flushing in the bonnet monkey
Data(s) |
01/04/1997
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Resumo |
A major limitation to progress in primate embryology is the lack of an adequate supply of preimplantation embryos. We describe a method for recovering preimplantation-embryos in bonnet monkeys (Macaca radiata ) using a nonsurgical uterine flushing technique similar to the one previously employed in rhesus monkeys. Forty cyclic females were screened for cervical cannulation, and 10% of these had an impassable cervix. Eleven females suitable for cannulation were selected, and 27 menstrual cycles were monitored over a 5-mo period. Seventy-one percent of the cycles showed estrogen peaks, which were observed between Days 9 and 14 of the cycle. Following natural mating, uterine flushings were performed on Days 5 to 8 of pregnancy (Day 0 = the day following the estrogen peak). Of the 27 recovery attempts, 9 (33.3%) resulted in the recovery of ovulation products, including those of an unfertilized oocyte and empty zona (2 cases), retarded cleavage-stage (4 to 8-cell) embryos (4 cases), morula (1 case) and blastocysts (2 cases). These results show, for the first time, that the nonsurgical uterine flushing technique can be successfully performed to recover uterine-stage preimplantation embryos from bonnet monkeys. |
Formato |
application/pdf |
Identificador |
Jayaprakash, D and Satish, KS and Ramachandra, SG and Ramesh, V and Seshagiri, PB (1997) Successful recovery of preimplantation embryos by nonsurgical uterine flushing in the bonnet monkey. In: Theriogenology, 47 (5). 1019 -1026. |
Publicador |
Elsevier Science |
Relação |
http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6TCM-3RJ9CF2-7&_user=512776&_coverDate=04%2F01%2F1997&_rdoc=1&_fmt=high&_orig=search&_sort=d&_docanchor=&view=c&_acct=C000025298&_version=1&_urlVersion=0&_userid=512776&md5=518d455af49b1d66d1cff56b http://eprints.iisc.ernet.in/24658/ |
Palavras-Chave | #Molecular Reproduction, Development & Genetics (formed by the merger of DBGL and CRBME) |
Tipo |
Journal Article PeerReviewed |