Isolation and characterization of 21 polymorphic microsatellite loci in the iconic Australian lungfish, Neoceratodus forsteri, using the Ion Torrent next-generation sequencing platform


Autoria(s): Huey, J. A.; Real, K. M.; Mather, P. B.; Chand, V.; Roberts, D. T.; Espinoza, T.; McDougall, A.; Kind, P. K.; Brooks, S.; Hughes, J. M.
Data(s)

2013

Resumo

We isolated and characterized 21 microsatellite loci in the vulnerable and iconic Australian lungfish, Neoceratodus forsteri. Loci were screened across eight individuals from the Burnett River and 40 individuals from the Pine River. Genetic diversity was low with between one and six alleles per locus within populations and a maximum expected heterozygosity of 0.774. These loci will now be available to assess effective population sizes and genetic structure in N. forsteri across its natural range in South East Queensland, Australia.

Identificador

Huey, J. A. and Real, K. M. and Mather, P. B. and Chand, V. and Roberts, D. T. and Espinoza, T. and McDougall, A. and Kind, P. K. and Brooks, S. and Hughes, J. M. (2013) Isolation and characterization of 21 polymorphic microsatellite loci in the iconic Australian lungfish, Neoceratodus forsteri, using the Ion Torrent next-generation sequencing platform. Conservation Genetics Resources, 5 (3). pp. 737-740. ISSN 1877-7252

http://era.daf.qld.gov.au/4450/

Relação

http://dx.doi.org/10.1007/s12686-013-9894-8

http://era.daf.qld.gov.au/4450/

Palavras-Chave #Fishery research #Fishery conservation
Tipo

Article

PeerReviewed