Freeze-drying of ovalbumin loaded mesoporous silica nanoparticle vaccine formulation increases antigen stability under ambient conditions
Data(s) |
2014
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Resumo |
Amino functionalised mesoporous silica nanoparticles (AM-41) have been identified as a promising vaccine delivery material. The capacity of AM-41 to stabilise vaccine components at ambient temperature (23–27 °C) was determined by adsorbing the model antigen ovalbumin (OVA) to AM-41 particles (OVA-41). The OVA-41 was successfully freeze-dried using the excipients 5% trehalose and 1% PEG8000. Both the immunological activity of OVA and the nanoparticle structure were maintained following two months storage at ambient temperature. The results of immunisation studies in mice with reconstituted OVA-41 demonstrated the induction of humoral and cell-meditated immune responses. The capacity of AM-41 particles to facilitate ambient storage of vaccine components without loss of immunological potency will underpin the further development of this promising vaccine delivery platform. |
Identificador |
Mody, Karishma T and Mahony, Donna and Cavallaro, Antonino S and Stahr, Frances and Qiao, Shi Zhang and Mahony, Timothy J and Mitter, Neena (2014) Freeze-drying of ovalbumin loaded mesoporous silica nanoparticle vaccine formulation increases antigen stability under ambient conditions. International Journal of Pharmaceutics . ISSN 0378-5173 |
Relação |
http://dx.doi.org/10.1016/j.ijpharm.2014.01.037 http://era.daf.qld.gov.au/4341/ |
Palavras-Chave | #Veterinary pharmacology |
Tipo |
Article PeerReviewed |