High-resolution melt-curve analysis of random amplified polymorphic DNA (RAPD-HRM) for the characterisation of pathogenic leptospires: intra-serovar divergence, interserovar convergence, and evidence of attenuation in Leptospira reference collections.
Data(s) |
2010
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Resumo |
High-resolution melt-curve analysis of random amplified polymorphic DNA (RAPD-HRM) is a novel technology that has emerged as a possible method to characterise leptospires to serovar level. RAPD-HRM has recently been used to measure intra-serovar convergence between strains of the same serovar as well as inter-serovar divergence between strains of different serovars. The results indicate that intra-serovar heterogeneity and inter-serovar homogeneity may limit the application of RAPD-HRM in routine diagnostics. They also indicate that genetic attenuation of aged, high-passage-number isolates could undermine the use of RAPD-HRM or any other molecular technology. Such genetic attenuation may account for a general decrease seen in titres of rabbit hyperimmune antibodies over time. Before RAPD-HRM can be further advanced as a routine diagnostic tool, strains more representative of the wild-type serovars of a given region need to be identified. Further, RAPD-HRM analysis of reference strains indicates that the routine renewal of reference collections, with new isolates, may be needed to maintain the genetic integrity of the collections. |
Identificador |
Tulsiani, S.M. and Craig, S.B. and Graham, G.C. and Cobbold, R.C. and Dohnt, M.F. and Burns, M.A. and Jansen, C.C. and Leung, L.K.P. and Field, H.E. and Smythe, L.D. (2010) High-resolution melt-curve analysis of random amplified polymorphic DNA (RAPD-HRM) for the characterisation of pathogenic leptospires: intra-serovar divergence, interserovar convergence, and evidence of attenuation in Leptospira reference collections. Annals of Tropical Medicine and Parasitology, 104 (5). pp. 427-437. |
Publicador |
W. S. Maney & Son Ltd |
Relação |
http://dx.doi.org/10.1179/136485910X12786389891047 http://era.daf.qld.gov.au/1828/ |
Palavras-Chave | #Science (General) #Genetics #Bacteria |
Tipo |
Article PeerReviewed |