A potential epigenetic marker mediating serum folate and vitamin B12 levels contributes to ischemic stroke risk
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01/02/2015
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Resumo |
Background and Purpose Stroke is a multifactorial disease that may be associated with aberrant DNA methylation profiles.We investigated epigenetic dysregulation for the MTHFR gene among ischaemic stroke patients. Methods Cases (n=297) and controls (n=110) were recruited after obtaining signed written informed consent, following a screening process against the inclusion/exclusion criteria. Serum vitamin metabolites (folate, vitamin B12 and homocysteine) were determined using immunoassays and methylation profiles for CpGs A and B in the MTHFR gene were determined using bisulfitepyrosequencing method. Results Methylation of MTHFR significantly increased the susceptibility risk for ischemic stroke. In particular, CpG A outperformed CpG B in mediating folate and vitamin B12 levels to increase ischemic stroke susceptibility risks by 4.73 fold. CpGs A and B were not associated with either serum homocysteine levels or ischemic stroke severity. Conclusion CpG A is a potential epigenetic marker in mediating serum folate and vitamin B12 to contribute to ischemic stroke. |
Formato |
application/pdf |
Identificador | |
Publicador |
Hindawi Publishing Corporation |
Relação |
http://eprints.qut.edu.au/90714/1/A%20potential%20epigenetic%20marker%20mediating%20serum%20folate%20and%20vitamin%20B12%20levels%20contributes%20to%20ischemic%20stroke%20risk_Wei.L.K.pdf http://www.hindawi.com/journals/bmri/2015/167976/ DOI:10.1155/2015/167976 Loo, Keat Wei, Griffiths, Lyn R., Sutherland, Heidi, Haupt, Larisa M., & Bhaskar, Shalini (2015) A potential epigenetic marker mediating serum folate and vitamin B12 levels contributes to ischemic stroke risk. BioMed Research International, 2015(167976). |
Direitos |
Copyright 2015 Loo KeatWei et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
Fonte |
School of Biomedical Sciences; Faculty of Health; Institute of Health and Biomedical Innovation |
Palavras-Chave | #060000 BIOLOGICAL SCIENCES #060400 GENETICS #060404 Epigenetics (incl. Genome Methylation and Epigenomics) #Ischemic Stroke #Epigenetics #Serum Folate #Vitamin B12 |
Tipo |
Journal Article |