Label-free isolation of a prostate cancer cell among blood cells and the single-cell measurement of drug accumulation using an integrated microfluidic chip
Data(s) |
17/10/2015
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Resumo |
Circulating tumor cells (CTCs) are found in the blood of patients with cancer. Although these cells are rare, they can provide useful information for chemotherapy. However, isolation of these rare cells from blood is technically challenging because they are small in numbers. An integrated microfluidic chip, dubbed as CTC chip, was designed and fabricated for conducting tumor cell isolation. As CTCs usually show multidrug resistance (MDR), the effect of MDR inhibitors on chemotherapeutic drug accumulation in the isolated single tumor cell is measured. As a model of CTC isolation, human prostate tumor cells were mixed with mouse blood cells and the labelfree isolation of the tumor cells was conducted based on cell size difference. The major advantages of the CTC chip are the ability for fast cell isolation, followed by multiple rounds of single-cell measurements, suggesting a potential assay for detecting the drug responses based on the liquid biopsy of cancer patients. |
Formato |
application/pdf |
Identificador | |
Publicador |
A I P Publishing LLC |
Relação |
http://eprints.qut.edu.au/89635/1/Revised%20CTC%20chip%20manuscript.pdf Khamenehfar, Avid, Beischlag, Timothy V., Russell, Pamela J., & Ling, Patrick (2015) Label-free isolation of a prostate cancer cell among blood cells and the single-cell measurement of drug accumulation using an integrated microfluidic chip. Biomicrofluidics. (In Press) |
Fonte |
School of Biomedical Sciences; Faculty of Health; Institute of Health and Biomedical Innovation |
Palavras-Chave | #100402 Medical Biotechnology Diagnostics (incl. Biosensors) #111201 Cancer Cell Biology #Circulating tumour cells #Human prostate cancer cells #Blood cells #multidrug resistance #microfluidic chip #same-single-cell analysis |
Tipo |
Journal Article |