Molecular and functional characterizations of a Kunitz-type serine protease inhibitor FcKuSPI of the shrimp Fenneropenaeus chinensis
Data(s) |
01/09/2013
|
---|---|
Resumo |
Serine proteinase inhibitors play important and diverse roles in biological processes such as coagulation, defense mechanisms, and immune responses. Here, we identified and characterized a Kunitz-type proteinase inhibitor, designated FcKuSPI, of the BPTI/Kunitz family of serine proteinase inhibitors from the hemocyte cDNA library of the shrimp Fenneropenaeus chinensis. The deduced amino acid sequence of FcKuSPI comprises 80 residues with a putative signal peptide of 15 amino acids. The predicted molecular weight of the mature peptide is 7.66 kDa and its predicted isoelectric point is 8.84. FcKuSPI includes a Kunitz domain containing six conserved cysteine residues that are predicted to form three disulfide bonds. FcKuSPI shares 44e53% homology with BPTI/Kunitz family members from other species. FcKuSPI mRNAwas expressed highly in the hemocytes and moderately in muscle in healthy shrimp. Recombinant FcKuSPI protein demonstrated anti-protease activity against trypsin and anticoagulant activity against citrated human plasma in a dose-dependent manner in in vitro assays. |
Identificador | |
Publicador |
Elsevier Academic Press |
Relação |
DOI:10.1016/j.fsi.2013.06.023 Kong, Hee Jeong, Lee, Ye-Ji, Park, In-Suk, Lee, Won Woo, Kim, Young-Ok, Nam, Bo-Hye, Kim, Woo-Jin, Jung, Hyungtaek, Jeon, You-Jin, An, Cheul Min, & Lee, Sang-Jun (2013) Molecular and functional characterizations of a Kunitz-type serine protease inhibitor FcKuSPI of the shrimp Fenneropenaeus chinensis. Fish and Shellfish Immunology, 35(3), pp. 1025-1029. |
Direitos |
Copyright 2013 Elsevier |
Fonte |
Centre for Tropical Crops and Biocommodities; School of Earth, Environmental & Biological Sciences; Institute for Future Environments; Science & Engineering Faculty |
Palavras-Chave | #Kunitz-type #Serine proteinase inhibitor #Fenneropenaeus chinensis #Hemocyte #Anticoagulant |
Tipo |
Journal Article |