N,N'-Carbonyldiimidazole-mediated functionalization of superparamagnetic nanoparticles as vaccine carrier
Data(s) |
2011
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Resumo |
Particulates with specific sizes and characteristics can induce potent immune responses by promoting antigen uptake of appropriate immuno-stimulatory cell types. Magnetite (Fe3O4) nanoparticles have shown many potential bioapplications due to their biocompatibility and special characteristics. Here, superparamagnetic Fe3O4 nanoparticles (SPIONs) with high magnetization value (70emug-1) were stabilized with trisodium citrate and successfully conjugated with a model antigen (ovalbumin, OVA) via N,N'-carbonyldiimidazole (CDI) mediated reaction, to achieve a maximum conjugation capacity at approximately 13μgμm-2. It was shown that different mechanisms governed the interactions between the OVA molecules and magnetite nanoparticles at different pH conditions. We evaluated as-synthesized SPION against commercially available magnetite nanoparticles. The cytotoxicity of these nanoparticles was investigated using mammalian cells. The reported CDI-mediated reaction can be considered as a potential approach in conjugating biomolecules onto magnetite or other biodegradable nanoparticles for vaccine delivery. |
Identificador | |
Publicador |
Elsevier BV |
Relação |
DOI:10.1016/j.colsurfb.2010.11.001 Ho, Jenny, Al-Deen, Fatin M. Nawwab, Al-Abboodi, Aswan, Selomulya, Cordelia, Xiang, Sue D., Plebanski, Magdalena, & Forde, Gareth M. (2011) N,N'-Carbonyldiimidazole-mediated functionalization of superparamagnetic nanoparticles as vaccine carrier. Colloids and Surfaces B : Biointerfaces, 83(1), pp. 83-90. |
Fonte |
School of Chemistry, Physics & Mechanical Engineering; Science & Engineering Faculty |
Palavras-Chave | #Magnetite #N #N'-Carbonyldiimidazole #Particulate vaccine carrier #Superparamagnetic nanoparticles #Bioapplications #Biodegradable nanoparticle #Carbonyldiimidazole #Cell types #Different mechanisms #Functionalizations #High magnetization #Immune response #Magnetite nanoparticles #Mammalian cells #Mediated reactions #Ovalbumins #pH condition #Superparamagnetics #Trisodium citrates #Vaccine carriers #Vaccine delivery #Antigens #Biocompatibility #Mammals #Superparamagnetism #Vaccines #Nanoparticles #citrate trisodium #drug carrier #imidazole derivative #ovalbumin #superparamagnetic iron oxide nanoparticle #unclassified drug #vaccine #adsorption kinetics #animal cell #article #cell viability #controlled study #cytotoxicity test #drug cytotoxicity #drug delivery system #hydrodynamics #hydrophilicity #isoelectric point #isotherm #light scattering #magnetic field #molecular interaction #molecular weight #nonhuman #particle size #pH #priority journal #scanning electron microscopy #transmission electron microscopy #zeta potential #Animals #Cell Death #Cell Survival #Cercopithecus aethiops #COS Cells #Drug Carriers #Electrophoresis #Polyacrylamide Gel #Ferric Compounds #Hydrogen-Ion Concentration #Imidazoles #Magnetics #Spectroscopy #Fourier Transform Infrared #Temperature #X-Ray Diffraction #Mammalia |
Tipo |
Journal Article |