Anti-Inflammatory and antiosteoclastogenic activities of parthenolide on human periodontal ligament cellsIn vitro
Data(s) |
2014
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Resumo |
Periodontitis is an inflammatory disease that causes osteolysis and tooth loss. It is known that the nuclear factor kappa B (NF-κB) signalling pathway plays a key role in the progression of inflammation and osteoclastogenesis in periodontitis. Parthenolide (PTL), a sesquiterpene lactone extracted from the shoots of Tanacetum parthenium, has been shown to possess anti-inflammatory properties in various diseases. In the study reported herein, we investigated the effects of PTL on the inflammatory and osteoclastogenic response of human periodontal ligament-derived cells (hPDLCs) and revealed the signalling pathways in this process. Our results showed that PTL decreased NF-κB activation, I-κB degradation, and ERK activation in hPDLCs. PTL significantly reduced the expression of inflammatory (IL-1β, IL-6, and TNF-α) and osteoclastogenic (RANKL, OPG, and M-CSF) genes in LPS-stimulated hPDLCs. In addition, PTL attenuated hPDLC-induced osteoclastogenic differentiation of macrophages (RAW264.7 cells), as well as reducing gene expression of osteoclast-related markers in RAW264.7 cells in an hPDLC-macrophage coculture model. Taken together, these results demonstrate the anti-inflammatory and antiosteoclastogenic activities of PTL in hPDLCs in vitro. These data offer fundamental evidence supporting the potential use of PTL in periodontitis treatment. |
Formato |
application/pdf |
Identificador | |
Publicador |
Hindawi Publishing |
Relação |
http://eprints.qut.edu.au/79796/1/79796.pdf DOI:10.1155/2014/546097 Zhang, Xufang, Fan, Chen, Xiao, Yin, & Mao, Xueli (2014) Anti-Inflammatory and antiosteoclastogenic activities of parthenolide on human periodontal ligament cellsIn vitro. Evidence-Based Complementary and Alternative Medicine, 2014, Article ID-546097. |
Direitos |
The authors Creative Commons Attribution Licence |
Fonte |
School of Chemistry, Physics & Mechanical Engineering; Institute of Health and Biomedical Innovation; Science & Engineering Faculty |
Tipo |
Journal Article |