Differential substrate behaviours of ethylene oxide and propylene oxide towards human glutathione transferase theta hGSTT1-1
Data(s) |
01/12/1999
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Resumo |
The transformation of ethylene oxide (EO), propylene oxide (PO) and 1- butylene oxide (1-BuO) by human glutathione transferase theta (hGSTT1-1) was studied comparatively using 'conjugator' (GSTT1 + individuals) erythrocyte lysates. The relative sequence of velocity of enzymic transformation was PO > EO >> 1-BuO. The faster transformation of PO compared to EO was corroborated in studies with human and rat GSTT1-1 (hGSTT1-1 and rGSTT1-1, respectively) expressed by Salmonella typhimurium TA1535. This sequence of reactivities of homologous epoxides towards GSTT1-1 contrasts to the sequence observed in homologous alkyl halides (methyl bromide, MBr; ethyl bromide, EtBr; n-propyl bromide, PrBr) where the relative sequence MeBr >> EtBr > PrBr is observed. The higher reactivity towards GSTT1-1 of propylene oxide compared to ethylene oxide is consistent with a higher chemical reactivity. This is corroborated by experimental data of acid-catalysed hydrolysis of a number of aliphatic epoxides, including ethylene oxide and propylene oxide and consistent with semi-empirical molecular orbital modelings. |
Identificador | |
Publicador |
Springer |
Relação |
DOI:10.1007/s002040050640 Thier, Ricarda, Wiebel, Frederike A., & Bolt, Hermann M. (1999) Differential substrate behaviours of ethylene oxide and propylene oxide towards human glutathione transferase theta hGSTT1-1. Archives of Toxicology, 73(8-9), pp. 489-492. |
Direitos |
Copyright 1999 Springer |
Fonte |
School of Clinical Sciences; Faculty of Health |
Palavras-Chave | #Butylene oxide #Ethylene oxide #Glutathione transferase theta #Methyl bromide #Propylene oxide #glutathione transferase #isoenzyme #organobromine derivative #recombinant enzyme #article #chemoreactivity #controlled study #degradation #hemolysate #human #human cell #nonhuman #priority journal #protein expression #reaction analysis #toxicokinetics #Animals #Carcinogens #Epoxy Compounds #Erythrocytes #Glutathione #Humans #Kinetics #Rats #Salmonella typhimurium |
Tipo |
Journal Article |