The Saccharomyces cerevisiae RNA-binding protein Rbp29 functions in cytoplasmic mRNA metabolism
Data(s) |
2000
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Resumo |
Here we report that the Saccharomyces cerevisiae RBP29 (SGN1, YIR001C) gene encodes a 29-kDa cytoplasmic protein that binds to mRNA in vivo. Rbp29p can be co-immunoprecipitated with the poly(A) tail-binding protein Pab1p from crude yeast extracts in a dosageand RNA-dependent manner. In addition, recombinant Rbp29p binds preferentially to poly(A) with nanomolar binding affinity in vitro. Although RBP29 is not essential for cell viability, its deletion exacerbates the slow growth phenotype of yeast strains harboring mutations in the eIF4G genes TIF4631 and TIF4632. Furthermore, overexpression of RBP29 suppresses the temperaturesensitive growth phenotype of specific tif4631, tif4632, and pab1 alleles. These data suggest that Rbp29p is an mRNA-binding protein that plays a role in modulating the expression of cytoplasmic mRNA. |
Formato |
application/pdf |
Identificador | |
Publicador |
The American Society for Biochemistry and Molecular Biology, Inc. |
Relação |
http://eprints.qut.edu.au/74848/1/J._Biol._Chem.-2000-Winstall-21817-26.pdf DOI:10.1074/jbc.M002412200 Winstall, Eric, Sadowski, Martin, Kuhn, Uwe, Wahle, Elmar, & Sachs, Alan B. (2000) The Saccharomyces cerevisiae RNA-binding protein Rbp29 functions in cytoplasmic mRNA metabolism. Journal of Biological Chemistry, 275(29), pp. 21817-21826. |
Direitos |
Copyright 2000 The American Society for Biochemistry and Molecular Biology, Inc. |
Fonte |
School of Biomedical Sciences; Faculty of Health; Institute of Health and Biomedical Innovation |
Tipo |
Journal Article |