Delivery of nitric oxide for analysis of the function of cytochrome c'


Autoria(s): Cole, Lindsay J.; Huston, Wilhelmina M.; Moir, James W.B.
Data(s)

2008

Resumo

On delivery of nitric oxide (NO) to protein samples (e.g., cytochrome c'), for spectroscopic experiments it is important to avoid exposure to oxygen and to remove contaminants from the NO gas. We describe a number of techniques for steady-state UV/Vis spectrophotometry and pre-steady-state stopped-flow spectrophotometry analysis of cytochrome c'.

Identificador

http://eprints.qut.edu.au/74467/

Publicador

Academic Press

Relação

DOI:10.1016/S0076-6879(08)36002-9

Cole, Lindsay J., Huston, Wilhelmina M., & Moir, James W.B. (2008) Delivery of nitric oxide for analysis of the function of cytochrome c'. Methods in Enzymology, 436, pp. 21-33.

Fonte

School of Biomedical Sciences; Faculty of Health; Institute of Health and Biomedical Innovation

Palavras-Chave #060100 BIOCHEMISTRY AND CELL BIOLOGY #steady-state UV/Vis #cytochrome c' #pre-steady-state stopped-flow #spectrophotometry
Tipo

Journal Article