Nucleic acid hybridizations as radioactive tool for rapid detection of banana bunchy top virus
Data(s) |
2013
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Resumo |
Banana bunchy top disease (BBTD) caused by banana bunchy top virus (BBTV) was radioactively detected by nucleic acid hybridization techniques. Results showed that, 32P-labelled insert of pBT338 was hybridized with nucleic acid extracts from BBTV-infected plants from Egypt and Australia but not with those from CMV-infected plants from Egypt. Results revealed that BBTV was greatly detected in midrib, roots, meristem, corm, leaves and pseudostem respectively. BBTV was also detected in symptomless young plants prepared from diseased plant materials grown under tissue culture conditions but was not present in those performed from healthy plant materials. The sensitivity of dot blot and Southern blot hybridizations for the detection of BBTV was also performed for the detection of BBTV. |
Identificador | |
Publicador |
Institute of Biotechnology and Genetic Engineering |
Relação |
http://pjbt.org/pdf/2013/P-4.pdf Allam, E.K., Dale, J.L., El-Afifi, S.I., Harding, R.M., & Sadik, A.S. (2013) Nucleic acid hybridizations as radioactive tool for rapid detection of banana bunchy top virus. Pakistan Journal of Biotechnology, 10(1), pp. 1-9. |
Fonte |
Centre for Tropical Crops and Biocommodities; School of Earth, Environmental & Biological Sciences; Science & Engineering Faculty |
Palavras-Chave | #Banana Bunchy Top Disease #Banana Bunchy Top Virus #Radioactive Detection #Southern and Dot Blot Hybridizations |
Tipo |
Journal Article |