Expression, regulation and putative nutrient-sensing function of taste GPCRs in the heart
Data(s) |
15/05/2013
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Resumo |
G protein-coupled receptors (GPCRs) are critical for cardiovascular physiology. Cardiac cells express >100 nonchemosensory GPCRs, indicating that important physiological and potential therapeutic targets remain to be discovered. Moreover, there is a growing appreciation that members of the large, distinct taste and odorant GPCR families have specific functions in tissues beyond the oronasal cavity, including in the brain, gastrointestinal tract and respiratory system. To date, these chemosensory GPCRs have not been systematically studied in the heart. We performed RT-qPCR taste receptor screens in rodent and human heart tissues that revealed discrete subsets of type 2 taste receptors (TAS2/Tas2) as well as Tas1r1 and Tas1r3 (comprising the umami receptor) are expressed. These taste GPCRs are present in cultured cardiac myocytes and fibroblasts, and are enriched in myocytes, which we corroborated using in situ hybridization. Tas1r1 gene-targeted mice (Tas1r1Cre/Rosa26tdRFP) strikingly recapitulated these data. In vivo taste receptor expression levels were developmentally regulated in the postnatal period. Intriguingly, several Tas2rs were upregulated in cultured rat myocytes and in mouse heart in vivo following starvation. The discovery of taste GPCRs in the heart opens an exciting new field of cardiac research. We predict that these taste receptors may function as nutrient sensors in the heart. |
Formato |
application/pdf application/pdf |
Identificador | |
Publicador |
Public Library of Science |
Relação |
http://eprints.qut.edu.au/68389/1/68389p.pdf http://eprints.qut.edu.au/68389/2/68389.pdf http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0064579 DOI:10.1371/journal.pone.0064579 Foster, Simon R., Porrello, Enzo R., Purdue, Brooke, Chan, Hsiu-Wen, Voigt, Anja, Frenzel, Sabine, Hannan, Ross D., Moritz, Karen M., Simmons, David G., Molenaar, Peter, Roura, Eugeni, Boehm, Ulrich, Meyerhof, Wolfgang, & Thomas, Walter G. (2013) Expression, regulation and putative nutrient-sensing function of taste GPCRs in the heart. PLoS ONE, 8(5), e64579. |
Direitos |
Copyright 2013 Foster et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
Fonte |
Faculty of Health; Institute of Health and Biomedical Innovation |
Palavras-Chave | #110201 Cardiology (incl. Cardiovascular Diseases) #111501 Basic Pharmacology #Taste Receptor #TAS1R #TAS2R #heart #cardiomyocyte #nutrient |
Tipo |
Journal Article |