Progesterone activates multiple innate immune pathways inChlamydia trachomatis-Infected endocervical cells
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2014
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Resumo |
Problem Susceptibility to Chlamydia trachomatis infection is increased by oral con- traceptives and modulated by sex hormones. We therefore sought to determine the effects of female sex hormones on the innate immune response to C. trachomatis infection. Method of study ECC-1 endometrial cells, pre-treated with oestradiol or progesterone, were infected with C. trachomatis and the host transcriptome analysed by Illumina Sentrix HumanRef-8 microarray. Primary endocervical epithe- lial cells, prepared at either the proliferative or secretory phase of the menstrual cycle, were infected with C. trachomatis and cytokine gene expression determined by quantitative RT-PCR analysis. Results Chlamydia trachomatis yield from progesterone-primed ECC-1 cells was significantly reduced compared with oestradiol-treated cells. Genes upregulated in progesterone-treated and Chlamydia-infected cells only included multiple CC and CXC chemokines, IL-17C, IL-29, IL-32, TNF-a, DEFB4B, LCN2, S100A7-9, ITGAM, NOD2, JAK1, IL-6ST, type I and II interferon receptors, numerous interferon-stimulated genes and STAT6. CXCL10, CXCL11, CX3CL1 and IL-17C, which were also upregu- lated in infected secretory-stage primary cells, and there was a trend towards higher levels of immune mediators in infected secretory-phase compared with proliferative-phase cells. Conclusion Progesterone treatment primes multiple innate immune pathways in hormone-responsive epithelial cells that could potentially increase resis- tance to chlamydial infection. |
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application/pdf |
Identificador | |
Publicador |
John Wiley & Sons |
Relação |
http://eprints.qut.edu.au/67817/2/67817.pdf DOI:10.1111/aji.12168 Wan, Charles, Latter, Joanna L., Amirshahi, Ashkan, Symonds, Ian, Finnie, Jane, Bowden, Nikola, Scott, Rodney J., Cunningham, Kelly A., Timms, Peter, & Beagley, Kenneth W. (2014) Progesterone activates multiple innate immune pathways inChlamydia trachomatis-Infected endocervical cells. American Journal of Reproductive Immunology, 71(2), pp. 165-177. http://purl.org/au-research/grants/NHMRC/401245 |
Direitos |
Copyright 2013 John Wiley & Sons Ltd |
Fonte |
School of Biomedical Sciences; Faculty of Health; Institute of Health and Biomedical Innovation |
Palavras-Chave | #110707 Innate Immunity #110799 Immunology not elsewhere classified #110802 Medical Infection Agents (incl. Prions) #Chlamydia #innate immunity #gene array #progesterone |
Tipo |
Journal Article |