Purification of particles of subterranean clover red leaf virus using an industrial-grade cellulase
Data(s) |
1984
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Resumo |
Particles of two isolates of subterranean clover red leaf virus were purified by a method in which infected plant tissue was digested with an industrial-grade cellulase, Celluclast® 2.0 L type X. The yields of virus particles using this enzyme were comparable with those obtained using either of two laboratory-grade cellulases, Cellulase type 1 (Sigma) and Driselase®. However, the specific infectivity or aphid transmissibility of the particles purified using Celluclast® was 10-100 times greater than those of preparations obtained using laboratory-grade cellulases or no enzyme. The main advantage of using Celluclast® is that at present in Australia its cost is only ca. 1% of laboratory-grade cellulases. |
Identificador | |
Publicador |
Elsevier BV |
Relação |
DOI:10.1016/0166-0934(84)90069-7 Waterhouse, Peter M. & Helms, K. (1984) Purification of particles of subterranean clover red leaf virus using an industrial-grade cellulase. Journal of Virological Methods, 8(4), pp. 321-329. |
Direitos |
Copyright 1984 Elsevier BV |
Fonte |
School of Earth, Environmental & Biological Sciences; Science & Engineering Faculty |
Palavras-Chave | #060400 GENETICS #060700 PLANT BIOLOGY #clover red leaf virus #enzyme immunoassay #higher plant #nonhuman #virus purification #Animal #Aphids #Cellulase #Comparative Study #Enzyme-Linked Immunosorbent Assay #Fabaceae #Glycoside Hydrolases #Plant Diseases #Plant Viruses #Plants #Medicinal |
Tipo |
Journal Article |